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Journal of Biomedical Optics

Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging
Author(s): Jingying Jiang; Matthias Boese; Paul H. Turner; Ruikang Wang
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Paper Abstract

By use of a Fourier transform infrared (FTIR) spectroscopic imaging technique, we examine the dynamic optical clearing processes occurring in hyperosmotically biocompatible agents penetrating into skin tissue in vitro. The sequential collection of images in a time series provides an opportunity to assess penetration kinetics of dimethyl sulphoxide (DMSO) and glycerol beneath the surface of skin tissue over time. From 2-D IR spectroscopic images and 3-D false color diagrams, we show that glycerol takes at least 30 min to finally penetrate the layer of epidermis, while DMSO can be detected in epidermis after only 4 min of being topically applied over stratum corneum sides of porcine skin. The results demonstrate the potential of a FTIR spectroscopic imaging technique as an analytical tool for the study of dynamic optical clearing effects when the bio-tissue is impregnated by hyperosmotically biocompatible agents such as glycerol and DMSO.

Paper Details

Date Published: 1 March 2008
PDF: 7 pages
J. Biomed. Opt. 13(2) 021105 doi: 10.1117/1.2899153
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Jingying Jiang, Tianjin Univ. (China)
Matthias Boese, Bruker Optik GmbH (Germany)
Paul H. Turner, Bruker Optics Ltd. (United Kingdom)
Ruikang Wang, Oregon Health & Science Univ. (United States)

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