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Journal of Biomedical Optics

Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue
Author(s): Matthew V. Schulmerich; Jacqueline H. Cole; Kathryn A. Dooley; Michael D. Morris; Jaclynn Kreider; Steven A. Goldstein
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Paper Abstract

The effect of optical clearing with glycerol on the Raman spectra of bone tissue acquired transcutaneously on right and left tibiae from four mice is studied. Multiple transcutaneous measurements are obtained from each limb; glycerol is then applied as an optical clearing agent, and additional transcutaneous measurements are taken. Glycerol reduces the noise in the raw spectra (p=0.0037) and significantly improves the cross-correlation between the recovered bone factor and the exposed bone measurement in a low signal-to-noise region of the bone spectra (p=0.0245).

Paper Details

Date Published: 1 March 2008
PDF: 7 pages
J. Biomed. Opt. 13(2) 021108 doi: 10.1117/1.2892687
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Matthew V. Schulmerich, Univ. of Michigan (United States)
Jacqueline H. Cole, Univ. of Michigan (United States)
Kathryn A. Dooley, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)
Jaclynn Kreider, Univ. of Michigan Medical School (United States)
Steven A. Goldstein, Univ. of Michigan Medical School (United States)

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