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Journal of Biomedical Optics

Two-wavelength carbon dioxide laser application for in-vitro blood glucose measurements
Author(s): Martina C. Meinke; Gerhard J. Müller; Hansjörg Albrecht; Christina Antoniou; H. Richter; Jürgen M. Lademann
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Paper Abstract

To develop a fast and easy clinical method for glucose measurements on whole blood samples, changes in glucose spectra are investigated varying temperature, glucose concentration, and solvent using attenuated total reflection Fourier transform infrared (ATR- FTIR) measurements. The results show a stability of the spectra at different temperatures and wavelength shifts of the absorption bands when water is replaced by blood. Because the ATR measurements are influenced by sedimentation of the red blood cells, a two-wavelength CO2 laser is used to determine the glucose concentration in whole blood samples. For this purpose, the first laser wavelength λ1 is tuned to the maximum of the glucose absorption band in blood at 1080 cm-1, and the second laser wavelength λ2 is tuned to 950 cm-1 for background measurements. The transmitted laser power through the optical cell containing the whole blood sample at λ1 and λ2 is used to determine the ratio. This signal correlates well with the glucose concentration in the whole blood samples. The CO2 laser measurement is too fast to be influenced by the red blood cell sedimentation, and will be a suitable method for glucose determination in whole blood.

Paper Details

Date Published: 1 January 2008
PDF: 6 pages
J. Biomed. Opt. 13(1) 014021 doi: 10.1117/1.2870093
Published in: Journal of Biomedical Optics Volume 13, Issue 1
Show Author Affiliations
Martina C. Meinke, Charité Universitätsmedizin Berlin (Germany)
Gerhard J. Müller, Charité Universitätsmedizin Berlin (Germany)
Hansjörg Albrecht, Laser-und Medizin-Technologie GmbH (Germany)
Christina Antoniou, Univ. of Athens (Greece)
H. Richter, Charité Universitätsmedizin Berlin (Germany)
Jürgen M. Lademann, Charité Universitätsmedizin Berlin (Germany)

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