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Journal of Biomedical Optics

Decreasing photobleaching by silver nanoparticles on metal surfaces: application to muscle myofibrils
Author(s): Priya Muthu; Ignacy Gryczynski; Zygmunt Karol Gryczynski; John M. Talent; Irina Akopova; Julian Borejdo
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Paper Abstract

Recently it has become possible to study single protein molecules in a cell. However, such experiments are plagued by rapid photobleaching. We recently showed that the interaction of fluorophores with localized surface plasmon polaritons (LSPs) induced in the metallic nanoparticles led to a substantial reduction of photobleaching. We now investigate whether the photobleaching could be further reduced when the excited fluorophore interacts with the LSP excited in the metallic nanoparticles resident on mirrored surface. As an example we use myofibrils, subcellular structures within skeletal muscle. We compare nanoparticle-enhanced fluorescence of myofibrils in the presence and in the absence of a mirrored surface. The proximity of the mirrored surface led to enhancement of fluorescence and to a decrease in fluorescent lifetime, much greater than that observed in the presence of nanoparticles alone. We think that the effect is caused by the near-field interactions between fluorophores and LSP, and between fluorophores and propagating surface plasmons (PSPs) produced in the metallic surface by the nanoparticles. Photobleaching is decreased because fluorescence enhancement enables illumination with a weaker laser beam and because the decrease in fluorescence lifetime minimizes the probability of oxygen attack during the time a molecule is in the exited state.

Paper Details

Date Published: 1 January 2008
PDF: 9 pages
J. Biomed. Opt. 13(1) 014023 doi: 10.1117/1.2854120
Published in: Journal of Biomedical Optics Volume 13, Issue 1
Show Author Affiliations
Priya Muthu, The Univ. of North Texas Health Science Ctr. (United States)
Ignacy Gryczynski, The Univ. of North Texas Health Science Ctr. (United States)
Zygmunt Karol Gryczynski, The Univ. of North Texas Health Science Ctr. (United States)
John M. Talent, The Univ. of North Texas Health Science Ctr. (United States)
Irina Akopova, The Univ. of North Texas Health Science Ctr. (United States)
Julian Borejdo


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