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Journal of Biomedical Optics • Open Access

Diagnosis of degenerative lesions of supraspinatus rotator cuff tendons by Fourier transform-Raman spectroscopy

Paper Abstract

The Fourier transform (FT)-Raman spectroscopy technique is used to assess the biochemical alterations that occur in the degenerative process of the rotator cuff supraspinatus tendon. The main alterations observed occur in the glycine, proline, hydroxyproline, cysteine, cistine, phenylalanine, tyrosine, collagen I and III, nucleic acid, lipids, glycosaminoglycans, and metalloproteinases bands. An increasing intensity for these bands is found in degenerated tendons, a finding well correlated with hyaline state and cellular activity. Statistical analysis (principal components analysis and clustering) shows a clear separation of the spectra into nonhyalinized and hyalinized clusters, which enables the construction of a binary diagnosis model based on logistic regression. Best diagnosis provided a sensitivity of 66.0% and a specificity of 74.7% with 79.6% concordant pairs. The discriminating power of the diagnostic test is assessed by computing the area under the receiving-operator characteristic curve (AUC), which indicates good accuracy (AUC=0.81). In principle, these results indicate that Raman spectroscopy can be used as an auxiliary aid to improve shoulder tendon surgery quality by guiding anchoring onto more healthy (nonhyaline) pieces of tendons. This should contribute to a decrease in the current high rerupture rate (13 to 68%) for this procedure.

Paper Details

Date Published: 1 January 2008
PDF: 10 pages
J. Biomed. Opt. 13(1) 014018 doi: 10.1117/1.2841017
Published in: Journal of Biomedical Optics Volume 13, Issue 1
Show Author Affiliations
Sergio Godoy Penteado, Univ. do Vale do Paraíba (Brazil)
Bianca Palma Fogazza, Univ. do Vale do Paraíba (Brazil)
Carolina da Silva Carvalho, Univ. do Vale do Paraíba (Brazil)
Emilia Angela Loschiavo Arisawa, Univ. do Vale do Paraíba (Brazil)
Mario Augusto da Silva Martins, Univ. do Vale do Paraíba (Brazil)
Airton Abr Martin, Univ. do Vale do Paraíba (Brazil)
Herculano da Martinho, Univ. do Vale do Paraíba (Brazil)

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