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Journal of Biomedical Optics

Two postprocessing techniques for the elimination of background autofluorescence for fluorescence lifetime imaging microscopy
Author(s): Phill B. Jones; Aneta Rozkalne; Melanie Meyer-Luehmann; Tara L. Spires-Jones; Alexandra Makarova; Anand T. N. Kumar; Oksana Berezovska; Brian J. Bacskai; Bradley T. Hyman
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Paper Abstract

The analysis of fluorescence lifetime imaging microscopy (FLIM) data under complex biological conditions can be challenging. Particularly, the presence of short-lived autofluorescent aggregates can confound lifetime measurements in fluorescence energy transfer (FRET) experiments, where it can become confused with the signal from exogenous fluorophores. Here we report two techniques that can be used to discriminate the contribution of autofluorescence from exogenous fluorphores in FLIM. We apply the techniques to transgenic mice that natively express yellow fluorescence protein (YFP) in a subset of cortical neurons and to histological slices of aged human brain tissue, where we study the misfolding of intracellular tau protein in the form of neurofibrillary tangles.

Paper Details

Date Published: 1 January 2008
PDF: 8 pages
J. Biomed. Opt. 13(1) 014008 doi: 10.1117/1.2837169
Published in: Journal of Biomedical Optics Volume 13, Issue 1
Show Author Affiliations
Phill B. Jones, Massachusetts General Hospital (United States)
Aneta Rozkalne, Massachusetts General Hospital (United States)
Melanie Meyer-Luehmann, Massachusetts General Hospital (United States)
Tara L. Spires-Jones, Massachusetts General Hospital (United States)
Alexandra Makarova, Massachusetts General Hospital (United States)
Anand T. N. Kumar, Massachusetts General Hospital (United States)
Oksana Berezovska, Massachusetts General Hospital (United States)
Brian J. Bacskai, Massachusetts General Hospital (United States)
Bradley T. Hyman, Massachusetts General Hospital (United States)


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