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Journal of Biomedical Optics

Three-dimensional real-time imaging of cardiac cell motions in living embryos
Author(s): Jian Lu; Francisco Pereira; Scott E. Fraser; Morteza Gharib
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Paper Abstract

While quantitative analysis of dynamic biological cell motions in vivo is of great biomedical interest, acquiring 3-D (plus time) information is difficult due to the lack of imaging tools with sufficient spatial and temporal resolution. A novel 3-D high-speed microscopic imaging system is developed to enable 3-D time series data acquisition, based on a defocusing technique (DDPIV). Depth coordinate Z is resolved by the triangular image patterns generated by a mask with three apertures forming an equilateral triangle. Application of this technique to microscale imaging is validated by calibration of targets spread over the image field. 1-µm fluorescent tracer particles are injected into the blood stream of 32 h post-fertilization developing zebrafish embryos to help describe cardiac cell motions. 3-D and velocity fields of cardiovascular blood flow and trajectories of heart-wall motions are obtained.

Paper Details

Date Published: 1 January 2008
PDF: 8 pages
J. Biomed. Opt. 13(1) 014006 doi: 10.1117/1.2830824
Published in: Journal of Biomedical Optics Volume 13, Issue 1
Show Author Affiliations
Jian Lu, California Institute of Technology (United States)
Francisco Pereira, Instituto Nazionale Per Studi Ed Esperienze di Architecttura Navale (Italy)
Scott E. Fraser, California Institute of Technology (United States)
Morteza Gharib, California Institute of Technology (United States)

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