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Journal of Biomedical Optics • Open Access

Quantitative biomarkers of stem cell differentiation based on intrinsic two-photon excited fluorescence
Author(s): William Rice; David L. Kaplan; Irene Georgakoudi

Paper Abstract

We present the use of two-photon excited fluorescence (TPEF) as a noninvasive means to monitor differentiation of human mesenchymal stem cells (hMSCs) into an adipogenic pathway relying entirely on endogenous sources of contrast. Specifically, we demonstrate that TPEF can be used to reveal quantitative differences in the biochemical status and the shape of differentiating and nondifferentiating stem cells in two-dimensional (2-D) cultures. We find that even in simple 2-D cultures, not all cells are undergoing differentiation at the same rate. Last, such noninvasive approaches may also ultimately allow for determination of the lineage toward which the cells are differentiating (e.g., fat versus bone). Thus, intrinsic TPEF imaging provides quantitative morphological and biochemical biomarkers associated with stem cell differentiation and could serve as an important enabling technology in tissue engineering applications.

Paper Details

Date Published: 1 November 2007
PDF: 3 pages
J. Biomed. Opt. 12(6) 060504 doi: 10.1117/1.2823019
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
William Rice, Tufts Univ. (United States)
David L. Kaplan, Tufts Univ. (United States)
Irene Georgakoudi, Tufts Univ. (United States)


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