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Journal of Biomedical Optics

Multimodality optical imaging of embryonic heart microstructure
Author(s): Ronit Yelin; Dvir Yelin; William Oh; Seok-Hyun Yun; Caroline Boudoux; Benjamin J. Vakoc; Brett E. Bouma; Guillermo J. Tearney
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Paper Abstract

Study of developmental heart defects requires the visualization of the microstructure and function of the embryonic myocardium, ideally with minimal alterations to the specimen. We demonstrate multiple endogenous contrast optical techniques for imaging the Xenopus laevis tadpole heart. Each technique provides distinct and complementary imaging capabilities, including: 1. 3-D coherence microscopy with subcellular (1 to 2 μm) resolution in fixed embryos, 2. real-time reflectance confocal microscopy with large penetration depth in vivo, and 3. ultra-high speed (up to 900 frames per second) that enables real-time 4-D high resolution imaging in vivo. These imaging modalities can provide a comprehensive picture of the morphologic and dynamic phenotype of the embryonic heart. The potential of endogenous-contrast optical microscopy is demonstrated for investigation of the teratogenic effects of ethanol. Microstructural abnormalities associated with high levels of ethanol exposure are observed, including compromised heart looping and loss of ventricular trabecular mass.

Paper Details

Date Published: 1 November 2007
PDF: 12 pages
J. Biomed. Opt. 12(6) 064021 doi: 10.1117/1.2822904
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Ronit Yelin, Massachusetts General Hospital (United States)
Dvir Yelin, Massachusetts General Hospital (United States)
William Oh, Massachusetts General Hospital (United States)
Seok-Hyun Yun, Massachusetts General Hospital (United States)
Caroline Boudoux, Massachusetts General Hospital (United States)
Benjamin J. Vakoc, Massachusetts General Hospital (United States)
Brett E. Bouma, Massachusetts General Hospital (United States)
Guillermo J. Tearney, Massachusetts General Hospital (United States)

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