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Journal of Biomedical Optics

Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo
Author(s): Philippe Rochon; Mercé Jourdain; Jacques Mangalaboyi; Francois Fourrier; Sylvie Soulie-Begu; Bruno Buys; Guy Dehlin; Jean Claude Lesage; Marie Christine Chambrin; Serge R. Mordon
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Paper Abstract

Our purpose is to evaluate intramucosal gastric pH video imaging by 2′,7′-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied in 10 pigs: five in the control group and five with respiratory acidosis induced through rebreathing. The intramucosal pH of the gastric wall is measured every 5 s and the results demonstrate a good correlation (pearson correlation=0.832) between blood gases pH measurements and pH measured with the video endocopic imaging system. Our results confirm the feasibility of using BCECF fluorescence pH imaging to measure intramucosal pH in vivo.

Paper Details

Date Published: 1 November 2007
PDF: 7 pages
J. Biomed. Opt. 12(6) 064014 doi: 10.1117/1.2821698
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Philippe Rochon, Ctr. Hospitalier Regional Univ de Lille (France)
Mercé Jourdain, Ctr. Hospitalier Regional Univ de Lille (France)
Jacques Mangalaboyi, Ctr. Hospitalier Regional Univ de Lille (France)
Francois Fourrier, Ctr. Hospitalier Regional Univ de Lille (France)
Sylvie Soulie-Begu, Univ. Montpellier I (France)
Bruno Buys, INSERM (France)
Guy Dehlin, INSERM (France)
Jean Claude Lesage, INSERM (France)
Marie Christine Chambrin, INSERM (France)
Serge R. Mordon, INSERM (France)

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