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Journal of Biomedical Optics

Multimodality imaging of T-cell hybridoma trafficking in collagen-induced arthritic mice: image-based estimation of the number of cells accumulating in mouse paws
Author(s): Shahriar S. Yaghoubi; Remi J. Creusot; Pritha Ray; C. Garrison Fathman; Sanjiv Gambhir
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Paper Abstract

Appropriate targeting of therapeutic cells is essential in adoptive cellular gene therapy (ACGT). Imaging cell trafficking in animal models and patients will guide development of ACGT protocols. Collagen type II (C-II)-specific T cell hybridomas are transduced with a lentivirus carrying a triple fusion reporter gene (TFR) construct consisting of a fluorescent reporter gene (RG), a bioluminescent RG (hRluc), and a positron emission tomography (PET) RG. Collagen-induced arthritic (CIA) mice are scanned with a bioluminescence imaging camera before and after implantation of various known cell quantities in their paws. Linear regression analysis yields equations relating two parameters of image signal intensity in mice paws to the quantity of hRluc expressing cells in the paws. Afterward, trafficking of intravenously injected cells is studied by quantitative analysis of bioluminescence images. Comparison of the average cell numbers does not demonstrate consistently higher accumulation of T-cell hybridomas in the paws with higher inflammation scores, and injecting more cells does not cause increased accumulation. MicroPET images illustrate above background signal in the inflamed paws and chest areas of CIA mice. The procedures described in this study can be used to derive equations for cells expressing other bioluminescent RGs and in other animal models.

Paper Details

Date Published: 1 November 2007
PDF: 11 pages
J. Biomed. Opt. 12(6) 064025 doi: 10.1117/1.2821415
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Shahriar S. Yaghoubi, Stanford Univ. (United States)
Remi J. Creusot, Stanford Univ. (United States)
Pritha Ray, Stanford Univ. (United States)
C. Garrison Fathman, Stanford Univ. (United States)
Sanjiv Gambhir, Stanford Univ. (United States)

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