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Journal of Biomedical Optics • Open Access

Characterization of natural carious lesions by fluorescence spectroscopy at 405-nm excitation wavelength

Paper Abstract

We aim to characterize natural caries enamel lesions by fluorescence spectroscopy. Sixty human samples with natural noncavitated caries lesions on smooth surfaces were selected and classified into three groups: dull, shiny, and brown lesions. All the samples were analyzed externally at the natural surface and after hemisectionig internally at the center of the lesion. The lesions were excited with a 405-nm InGaN diode laser and the fluorescence was collected with a single grating spectrometer. Four emission bands (455, 500, 582, and 622 nm) are identified in both sound and carious regions. The area under each emission band is correlated with the total area of the four bands for the sound and carious regions. The detected fluorescence from natural and cut surfaces through the caries lesions is not statistically different for the shiny and dull lesion, but is different [analysis of variance (ANOVA) (p<0.05)] for brown lesion at all emission bands. At the 405-nm excitation wavelength, the area of the fluorescence bands at 455 and 500 nm differ statistically for natural carious lesions and sound tissue.

Paper Details

Date Published: 1 November 2007
PDF: 5 pages
J. Biomed. Opt. 12(6) 064013 doi: 10.1117/1.2821192
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Denise Maria Zezell, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Adriana Costa Ribeiro, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Luciano Bachmann, Univ. de São Paulo (Brazil)
Anderson Stevens Leônidas Gomes, Univ. Federal de Pernambuco (Brazil)
Christel Rousseau, Univ. of Strathclyde (United Kingdom)
John M. Girkin, Univ. of Strathclyde (United Kingdom)

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