Share Email Print
cover

Journal of Biomedical Optics

Time dependence of singlet oxygen luminescence provides an indication of oxygen concentration during oxygen consumption
Author(s): Jürgen Baier; Tim Maisch; Johannes Regensburger; Maria Loibl; Rudolf Vasold; Wolfgang Baeumler
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Singlet oxygen plays a major role in photodynamic inactivation of tumor cells or bacteria. Its efficacy depends critically on the oxygen concentration [O2], which can decrease in case oxygen is consumed caused by oxidative reactions. When detecting singlet oxygen directly by its luminescence at 1270 nm, the course of the luminescence signal is critically affected by [O2]. Thus, it should be feasible to monitor oxygen consumption during photo-oxidative processes. Singlet oxygen was generated by exciting a photosensitizer (TMPyP) in aqueous solution (H2O or D2O) of albumin. Chromatography shows that most of the TMPyP molecules are unbound, and therefore singlet oxygen molecules can diffuse in the solution. A sensor device for oxygen concentration revealed a rapid decrease of [O2] (oxygen depletion) in the solution during irradiation. The extent of oxygen depletion in aqueous albumin solution depends on the radiant exposure and the solvent. When detecting the luminescence signal of singlet oxygen, the shape of the luminescence signal significantly changed with irradiation time. Thus, local oxygen consumption could be monitored during photodynamic action by evaluating the course of singlet oxygen luminescence.

Paper Details

Date Published: 1 November 2007
PDF: 7 pages
J. Biomed. Opt. 12(6) 064008 doi: 10.1117/1.2821153
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Jürgen Baier, Univ. Regensburg (Germany)
Tim Maisch, Univ. Regensburg (Germany)
Johannes Regensburger, Univ. Regensburg (Germany)
Maria Loibl, Univ. Regensburg (Germany)
Rudolf Vasold, Univ. Regensburg (Germany)
Wolfgang Baeumler, Univ. Regensburg (Germany)


© SPIE. Terms of Use
Back to Top