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Journal of Biomedical Optics

Effect of light losses of sample between two integrating spheres on optical properties estimation
Author(s): Dan Zhu; Wei Lu; Shaoqun Zeng; Qingming Luo
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Paper Abstract

A Monte Carlo algorithm is applied to simulate the measurements of a sample with glass slides sandwiched between the double integrating sphere (DIS) setup. The effects caused by various parameters, such as the sample port of integrating sphere, thicknesses, and optical properties of the sample, on light losses and optical properties estimated by the inverse adding-doubling method (IAD) have been investigated. The results show that the light loss greatly increases the estimated error of absorption coefficient and slightly affects the estimated scattering coefficient. When the increase of apparent absorption of the sample induced by the light loss is 59%, the relative error of the scattering coefficient is less than 2% and that of the absorption coefficient reaches 28%. Enhancing the sample port diameter or decreasing the thickness of the sample can reduce the error effectively, and the effect of the former is much greater than that of the latter. In addition, the IAD method is proved to be valid for estimating the optical properties of a highly scattering or highly absorbing sample. This study can not only evaluate the error of optical properties estimation, but also provide optimal ways for the design of DIS and a scheme for acquiring accurate optical properties of tissue.

Paper Details

Date Published: 1 November 2007
PDF: 8 pages
J. Biomed. Opt. 12(6) 064004 doi: 10.1117/1.2815691
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Dan Zhu, Huazhong Univ. of Science and Technology (China)
Wei Lu, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)

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