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Journal of Biomedical Optics

Extended depth-of-focus microscopy via constrained deconvolution
Author(s): Jose-Angel Conchello; Michael Dresser
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Paper Abstract

We derive a method for extended depth-of-focus imaging, i.e., a method to render a 2-D image of a thick specimen, such that all the structures within the specimen appear in focus and with greatly increased contrast. We acquire a single image while moving the specimen through focus. The resulting image, which is severely blurred and has very low contrast, is then deconvolved. In the deconvolved image, the entire depth of the specimen is in focus. Because the image is collected continuously while the specimen moves through focus, the acquisition time is short. Likewise, because the deconvolution is done in 2-D, it is done very quickly even with an iterative algorithm.

Paper Details

Date Published: 1 November 2007
PDF: 7 pages
J. Biomed. Opt. 12(6) 064026 doi: 10.1117/1.2812554
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Jose-Angel Conchello, Oklahoma Medical Research Foundation (United States)
Michael Dresser, Oklahoma Medical Research Foundation (United States)

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