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Journal of Biomedical Optics

Optimized autofluorescence bronchoscopy using additional backscattered red light
Author(s): Tanja Gabrecht; Thomas M. Glanzmann; Lutz Freitag; Bernd-Claus Weber; Hubert van den Bergh; Georges A. Wagnières
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Paper Abstract

Autofluorescence bronchoscopy (AFB) has been shown to be a highly sensitive tool for the detection of early endobronchial cancers. When excited with blue-violet light, early neoplasia in the bronchi tend to show a decrease of autofluorescence in the green region of the spectrum and a relatively smaller decrease in the red region of the spectrum. Superposing the green foreground image and the red background image creates the resultant autofluorescence image. Our aim was to investigate whether the addition of backscattered red light to the tissue autofluorescence signal could improve the contrast between healthy and diseased tissue. We have performed a clinical study involving 41 lung cancers using modified autofluorescence bronchoscopy systems. The lesions were examined sequentially with conventional violet autofluorescence excitation (430 nm±30 nm) and violet autofluorescence excitation plus backscattered red light (430 nm±40 nm plus 665 nm±15 nm). The contrast between (pre-)neoplastic and healthy tissue was quantified with off-line image analysis. We observed a 2.7 times higher contrast when backscattered red light was added to the violet excitation. In addition, the image quality was improved in terms of the signal-to-noise ratio (SNR) with this spectral design.

Paper Details

Date Published: 1 November 2007
PDF: 9 pages
J. Biomed. Opt. 12(6) 064016 doi: 10.1117/1.2811952
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Tanja Gabrecht, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Thomas M. Glanzmann, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Lutz Freitag, Lungenklinik Hemer (Germany)
Bernd-Claus Weber, Richard Wolf GmbH (Germany)
Hubert van den Bergh, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Georges A. Wagnières, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

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