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Journal of Biomedical Optics

Noninvasive time-dependent cytometry monitoring by digital holography
Author(s): Manuel Kemmler; Markus Fratz; Dominik M. Giel; Norbert Saum; Albrecht Brandenburg; Christian Hoffmann
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Paper Abstract

Using a digital holographic microscope setup, it is possible to measure dynamic volume changes in living cells. The cells were investigated time-dependently in transmission mode for different kinds of stimuli affecting their morphology. The measured phase shift was correlated to the cellular optical thickness, and then of the cell volume as well as the refractive index were calculated and interpreted. For the characterization of the digital holographic microscope setup, we have developed a transparent three-dimensional (3-D) reference chart that can be used as a lateral resolution chart and step-height resolution chart included in one substrate. For the monitoring of living cells, a biocompatible and autoclavable flow chamber was designed, which allows us to add, exchange, or dilute the fluid within the flow chamber. An integrated changeable coverslip enables inverse microscopic applications. Trypsinization, cell swelling and shrinking induced by osmolarity changes, and apoptosis served as model processes to elucidate the potential of the digital holographic microscopy (DHM).

Paper Details

Date Published: 1 November 2007
PDF: 10 pages
J. Biomed. Opt. 12(6) 064002 doi: 10.1117/1.2804926
Published in: Journal of Biomedical Optics Volume 12, Issue 6
Show Author Affiliations
Manuel Kemmler, Fraunhofer-Institut für Physik Meßtechnik (Germany)
Markus Fratz, Fraunhofer-Institut für Physik Messtechnik (Germany)
Dominik M. Giel, Fraunhofer-Institut für Physik Messtechnik (Germany)
Norbert Saum, Fraunhofer-Institut für Physik Meßtechnik (Germany)
Albrecht Brandenburg, Fraunhofer-Institut für Physik Messtechnik (Germany)
Christian Hoffmann, Institut fur Bioprozess- und Analysenmesstechnik e.V. (Germany)

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