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Journal of Biomedical Optics

Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source
Author(s): Hiroyuki Yokoyama; Hiroshi Tsubokawa; Hengchang Guo; Junichi Shikata; Ki-ichi Sato; Keijiro Takashima; Kaori Kashiwagi; Naoaki Saito; Hirokazu Taniguchi; Hiromasa Ito
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Paper Abstract

We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

Paper Details

Date Published: 1 September 2007
PDF: 5 pages
J. Biomed. Opt. 12(5) 054019 doi: 10.1117/1.2800393
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Hiroyuki Yokoyama, Tohoku Univ. (Japan)
Hiroshi Tsubokawa, Tohoku Univ. (Japan)
Hengchang Guo, Tohoku Univ. (Japan)
Junichi Shikata, Tohoku Univ. (Japan)
Ki-ichi Sato, Tohoku Univ. (Japan)
Keijiro Takashima, Tohoku Univ. (Japan)
Kaori Kashiwagi, Kobe Univ. (Japan)
Naoaki Saito, Kobe Univ. (Japan)
Hirokazu Taniguchi, Tohoku Univ. (Japan)
Hiromasa Ito, The Institute of Physical and Chemical Research (RIKEN) (Japan)

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