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Journal of Biomedical Optics • Open Access

Subcellular imaging of epithelium with time-lapse optical coherence tomography
Author(s): Yingtian Pan; Z. L. Wu; Zhijia Yuan; Z. G. Wang; C. W. Du

Paper Abstract

We present the first experimental result of direct delineation of the nuclei of living rat bladder epithelium with ultrahigh-resolution optical coherence tomography (uOCT). We demonstrate that the cellular details embedded in the speckle noise in a uOCT image can be uncovered by time-lapse frame averaging that takes advantage of the micromotion in living biological tissue. The uOCT measurement of the nuclear size (7.9±1.4 μm) closely matches the histological evaluation (7.2±0.8 μm). Unlike optical coherence microscopy (OCM), which requires a sophisticated high-NA microscopic objective, this approach uses a commercial-grade single achromatic lens (f/10 mm, NA/0.25) and provides a cross-sectional image over 0.6 mm of depth without focus tracking, thus holding great promise of endoscopic optical biopsy for diagnosis and grading of flat epithelial cancer such as carcinoma in situ in vivo.

Paper Details

Date Published: 1 September 2007
PDF: 3 pages
J. Biomed. Opt. 12(5) 050504 doi: 10.1117/1.2800007
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Yingtian Pan, Stony Brook Univ. (United States)
Z. L. Wu, Stony Brook Univ. (United States)
Zhijia Yuan, State Univ. of New York/Stony Brook (United States)
Z. G. Wang, Stony Brook Univ. (United States)
C. W. Du, Stony Brook Univ. (United States)

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