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Journal of Biomedical Optics

Second harmonic generation imaging microscopy studies of osteogenesis imperfecta
Author(s): Oleg Nadiarnykh; Sergey Plotnikov; William A. Mohler; Ivo Kalajzic; Deborah Redford-Badwal; Paul J. Campagnola
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Paper Abstract

We have used quantitative second harmonic generation (SHG) imaging microscopy to investigate the collagen matrix organization in the oim mouse model for human osteogenesis imperfecta (OI). OI is a heritable disease in which the type I collagen fibrils are either abnormally organized or small, resulting in a clinical presentation of recurrent bone fractures and other pathologies related to collagen-comprised tissues. Exploiting the exquisite sensitivity of SHG to supramolecular assembly, we investigated whether this approach can be utilized to differentiate normal and oim tissues. By comparing SHG intensity, fibrillar morphology, polarization anisotropy, and signal directionality, we show that statistically different results are obtained for the wild type (WT) and disease states in bone, tendon, and skin. All these optical signatures are consistent with the collagen matrix in the oim tissues being more disordered, and these results are further consistent with the known weaker mechanical properties of the oim mouse. While the current work shows the ability of SHG to differentiate normal and diseased states in a mouse model, we suggest that our results provide a framework for using SHG as a clinical diagnostic tool for human OI. We further suggest that the SHG metrics described could be applied to other connective tissue disorders that are characterized by abnormal collagen assembly.

Paper Details

Date Published: 1 September 2007
PDF: 9 pages
J. Biomed. Opt. 12(5) 051805 doi: 10.1117/1.2799538
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Oleg Nadiarnykh, Univ. of Connecticut Health Ctr. (United States)
Sergey Plotnikov, Univ. of Connecticut Health Ctr. (United States)
William A. Mohler, Univ. of Connecticut Health Ctr. (United States)
Ivo Kalajzic, Univ. of Connecticut Health Ctr. (United States)
Deborah Redford-Badwal, Univ. of Connecticut Health Ctr. (United States)
Paul J. Campagnola, Univ. of Connecticut Health Ctr. (United States)

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