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Journal of Biomedical Optics

Reactive biomolecular divergence in genetically altered yeast cells and isolated mitochondria as measured by biocavity laser spectroscopy: rapid diagnostic method for studying cellular responses to stress and disease
Author(s): Paul Lee Gourley; Judy Kay Hendricks; Anthony E. McDonald; R. G. Copeland; Michael P. Yaffe; Robert K. Naviaux
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Paper Abstract

We report an analysis of four strains of baker's yeast (Saccharomyces cerevisiae) using biocavity laser spectroscopy. The four strains are grouped in two pairs (wild type and altered), in which one strain differs genetically at a single locus, affecting mitochondrial function. In one pair, the wild-type ρ+ and a ρ0 strain differ by complete removal of mitochondrial DNA (mtDNA). In the second pair, the wild-type ρ+ and a ρ- strain differ by knock-out of the nuclear gene encoding Co×4, an essential subunit of cytochrome c oxidase. The biocavity laser is used to measure the biophysical optic parameter Δλ, a laser wavelength shift relating to the optical density of cell or mitochondria that uniquely reflects its size and biomolecular composition. As such, Δλ is a powerful parameter that rapidly interrogates the biomolecular state of single cells and mitochondria. Wild-type cells and mitochondria produce Gaussian-like distributions with a single peak. In contrast, mutant cells and mitochondria produce leptokurtotic distributions that are asymmetric and highly skewed to the right. These distribution changes could be self-consistently modeled with a single, log-normal distribution undergoing a thousand-fold increase in variance of biomolecular composition. These features reflect a new state of stressed or diseased cells that we call a reactive biomolecular divergence (RBD) that reflects the vital interdependence of mitochondria and the nucleus.

Paper Details

Date Published: 1 September 2007
PDF: 14 pages
J. Biomed. Opt. 12(5) 054003 doi: 10.1117/1.2799198
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Paul Lee Gourley, Sandia National Labs. (United States)
Judy Kay Hendricks, Sandia National Labs. (United States)
Anthony E. McDonald, Sandia National Labs. (United States)
R. G. Copeland, Sandia National Labs. (United States)
Michael P. Yaffe, Univ. of California/San Diego (United States)
Robert K. Naviaux, Univ. of California/San Diego (United States)


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