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Journal of Biomedical Optics

Experimental parameters influencing surface-enhanced Raman scattering of bacteria
Author(s): Mehmet Kahraman; M.Muge Yazici; Fikrettin Sahin; Mustafa Culha
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Paper Abstract

Surface-enhanced Raman scattering (SERS) is a powerful technique for the analysis of a variety of molecules and molecular structures. Due to its great complexity, the acquisition of detailed molecular information from biological organizations such as bacteria is still a challenging task. SERS can provide valuable information once silver or gold surfaces can be brought in close contact with the biological organization. Because several experimental parameters can affect SERS spectra of bacteria, the experimental conditions must be well defined for comparable and reproducible results. The influence of experimental parameters, such as the type of noble metal, size, and aggregation properties of nanoparticles, and the wavelength of the laser light on the SERS of E. coli and B. megaterium are examined. It is demonstrated that the impact of these parameters could be enormous and a standard protocol must be developed depending on the goal of the study.

Paper Details

Date Published: 1 September 2007
PDF: 6 pages
J. Biomed. Opt. 12(5) 054015 doi: 10.1117/1.2798640
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Mehmet Kahraman, Yeditepe Univ. (Turkey)
M.Muge Yazici, Yeditepe Univ. (Turkey)
Fikrettin Sahin, Yeditepe Univ. (Turkey)
Mustafa Culha, Yeditepe Univ. (Turkey)

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