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Journal of Biomedical Optics

Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy
Author(s): Björn Kemper; Sebastian Kosmeier; Patrik Langehanenberg; Gert von Bally; Ilona Bredebusch; Wolfram Domschke; Jürgen Schnekenburger
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Paper Abstract

A method for the determination of the integral refractive index of living cells in suspension by digital holographic microscopy is described. Digital holographic phase contrast images of spherical cells in suspension are recorded, and the radius as well as the integral refractive index are determined by fitting the relation between cell thickness and phase distribution to the measured phase data. The algorithm only requires information about the refractive index of the suspension medium and the image scale of the microscope system. The specific digital holographic microscopy advantage of subsequent focus correction allows a simultaneous investigation of cells in different focus planes. Results obtained from human pancreas and liver tumor cells show that the integral cellular refractive index decreases with increasing cell radius.

Paper Details

Date Published: 1 September 2007
PDF: 5 pages
J. Biomed. Opt. 12(5) 054009 doi: 10.1117/1.2798639
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Björn Kemper, Univ. Münster (Germany)
Sebastian Kosmeier, Univ. Münster (Germany)
Patrik Langehanenberg, Univ. Münster (Germany)
Gert von Bally, Univ. Münster (Germany)
Ilona Bredebusch, Univ. Münster (Germany)
Wolfram Domschke, Univ. Münster (Germany)
Jürgen Schnekenburger, Univ. Münster (Germany)

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