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Journal of Biomedical Optics • Open Access

Confocal laser scanning microscopy of retinal rod outer segment intact disks: new labeling technique
Author(s): Silvia Ravera; Daniela Calzia; Paolo Bianchini; Alberto Diaspro; Isabella Panfoli

Paper Abstract

Vertebrate retinal rod outer segment disks house the proteins involved in the phototransduction cascade that converts light into neuronal signal. We develop a technique for the immunofluorescent labeling of osmotically intact isolated rod outer segment disks for confocal laser scanning microscopy imaging. Osmotically intact Ficoll-flotation isolated bovine disks are directly labeled with antibodies in solution. For the first time, osmotically intact single disks can be visualized. Thus, imaging of purified disks, based on advanced optical techniques, may serve as a powerful complement to other methods in studies on phototransduction. In fact, even though much is known about the rod outer segment photoresponse, some unanswered questions remain, particularly about ATP supply, light adaptation, and morphogenesis.

Paper Details

Date Published: 1 September 2007
PDF: 3 pages
J. Biomed. Opt. 12(5) 050501 doi: 10.1117/1.2790919
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Silvia Ravera, Univ. degli Studi di Genova (Italy)
Daniela Calzia, Univ. degli Studi di Genova (Italy)
Paolo Bianchini, Univ. degli Studi di Genova (Italy)
Alberto Diaspro, Univ. degli Studi di Genova (Italy)
Isabella Panfoli, Univ. degli Studi di Genova (Italy)

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