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Journal of Biomedical Optics

Noninvasive measurement of three-dimensional morphology of adhered animal cells employing phase-shifting laser microscope
Author(s): Mutsumi Takagi; Takayuki Kitabayashi; Syunsuke Ito; Masashi Fujiwara; Akio Tokuda
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Paper Abstract

Noninvasive measurement of 3-D morphology of adhered animal cells employing a phase-shifting laser microscope (PLM) is investigated, in which the phase shift for each pixel in the view field caused by cell height and the difference in refractive indices between the cells and the medium is determined. By employing saline with different refractive indices instead of a culture medium, the refractive index of the cells, which is necessary for the determination of cell height, is determined under PLM. The observed height of Chinese hamster ovary (CHO) cells cultivated under higher osmolarity is lower than that of the cells cultivated under physiological osmolarity, which is in agreement with previous data observed under an atomic force microscope (AFM). Maximum heights of human bone marrow mesenchymal stem cells and human umbilical cord vein endothelial cells measured under PLM and AFM agree well with each other. The maximum height of nonadherent spherical CHO cells observed under PLM is comparable to the cell diameter measured under a phase contrast inverted microscope. Laser irradiation, which is necessary for the observation under PLM, did not affect 3-D cell morphology. In conclusion, 3-D morphology of adhered animal cells can be noninvasively measured under PLM.

Paper Details

Date Published: 1 September 2007
PDF: 5 pages
J. Biomed. Opt. 12(5) 054010 doi: 10.1117/1.2779350
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Mutsumi Takagi, Hokkaido Univ. (Japan)
Takayuki Kitabayashi, Hokkaido Univ. (Japan)
Syunsuke Ito, Hokkaido Univ. (Japan)
Masashi Fujiwara, Hokkaido Univ. (Japan)
Akio Tokuda

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