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Journal of Biomedical Optics

In vivo dynamic light scattering imaging of blood coagulation
Author(s): Vyacheslav Kalchenko; Alexander G. Brill; Michael Bayewitch; Ilya Fine; Vladimir P. Zharov; Ekaterina I. Galanzha; Valery Viktorovich Tuchin; Alon Harmelin
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Paper Abstract

Physiological blood coagulation/clotting is an essential biological process that is initiated by vessel injury and includes a cascade of enzymatic reactions finalized by fibrin polymerization and clot formation. We utilize dynamic light scattering (DLS) imaging to monitor in vivo red cell mobility as an indicator of blood coagulation. In the course of the experiments, blood flow is arrested using mechanical occlusion, and then laser injury is applied. We demonstrate that the combination of laser injury with DLS imaging on occluded blood vessels (i.e., under static conditions) is suitable to detect even subtle changes of plasma viscosity in the circulatory system, which reflects the process of clot development. This approach is noninvasive and has a relatively simple and easy-to-use technical design. Thus, the proposed methodology provides a promising tool for investigating blood clotting within the vasculature.

Paper Details

Date Published: 1 September 2007
PDF: 4 pages
J. Biomed. Opt. 12(5) 052002 doi: 10.1117/1.2778695
Published in: Journal of Biomedical Optics Volume 12, Issue 5
Show Author Affiliations
Vyacheslav Kalchenko, Weizmann Institute of Science (Israel)
Alexander G. Brill, Hadassah Hebrew Univ. Medical Ctr. (Israel)
Michael Bayewitch, ELFI-Tech Ltd. (Israel)
Ilya Fine, ELFI-Tech Ltd. (Israel)
Vladimir P. Zharov, Univ. of Arkansas for Medical Sciences (United States)
Ekaterina I. Galanzha, Univ. of Arkansas for Medical Sciences (United States)
Valery Viktorovich Tuchin, Saratov State Univ. (Russia)
Alon Harmelin, Weizmann Institute of Science (Israel)

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