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Journal of Biomedical Optics

Indocyanine-green-embedded PEBBLEs as a contrast agent for photoacoustic imaging
Author(s): Gwangseong Kim; Sheng-Wen Huang; Kathleen C. Day; Matthew O'Donnell; Rodney R. Agayan; Mark A. Day; Raoul Kopelman; Shai Ashkenazi
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Paper Abstract

Nanoparticles 100 nm in diameter containing indocyanine green (ICG) have been developed as a contrast agent for photoacoustic (PA) imaging based on (photonic explorers for biomedical use by biologically localized embedding PEBBLE) technology using organically modified silicate (ormosil) as a matrix. ICG is an FDA-approved dye with strong optical absorption in the near-infrared (NIR) region, where light can penetrate deepest into biological tissue. A photoacoustic imaging system was used to study image contrast as a function of PEBBLE concentration in phantom objects. ICG-embedded ormosil PEBBLEs showed improved stability in aqueous solution compared with free ICG dye. The particles were conjugated with HER-2 antibody for breast cancer and prostate cancer cell targeting. Initial in vitro characterization shows high contrast and high efficiency for binding to prostate cancer cells. ICG can also be used as a photosensitizer (generating toxic oxygen by illumination) for photodynamic therapy. We have measured the photosensitization capability of ICG-embedded ormosil nanoparticles. This feature can be utilized to combine detection and therapeutic functions in a single agent.

Paper Details

Date Published: 1 July 2007
PDF: 8 pages
J. Biomed. Opt. 12(4) 044020 doi: 10.1117/1.2771530
Published in: Journal of Biomedical Optics Volume 12, Issue 4
Show Author Affiliations
Gwangseong Kim, Univ. of Michigan (United States)
Sheng-Wen Huang, Univ. of Michigan (United States)
Kathleen C. Day, Univ. of Michigan (United States)
Matthew O'Donnell, Univ. of Washington (United States)
Rodney R. Agayan, Univ. of Michigan (United States)
Mark A. Day, Univ. of Michigan (United States)
Raoul Kopelman, Univ. of Michigan (United States)
Shai Ashkenazi, Univ. of Michigan (United States)

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