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Journal of Biomedical Optics

Ultrahigh-resolution imaging of human donor cornea using full-field optical coherence tomography
Author(s): Masahiro Akiba; Naoyuki Maeda; Kazuhiko Yumikake; Takeshi Soma; Kohji Nishida; Yasuo Tano; Kin-Pui Chan
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Paper Abstract

A feasibility study of ultrahigh-resolution full-field optical coherence tomography (FF-OCT) for a subcellular-level imaging of human donor corneas is presented. The FF-OCT system employed in this experiment is based on a white light interference microscope, where the sample is illuminated by a thermal light source and a horizontal cross-sectional (en face) image is detected using a charge coupled device (CCD) camera. A conventional four-frame phase-shift detection technique is employed to extract the interferometric image from the CCD output. A 95-nm-broadband full-field illumination yields an axial resolution of 2.0 μm, and the system covers an area of 850 μm×850 μm with a transverse resolution of 2.4 μm using a 0.3-NA microscope objective and a CCD camera with 512×512 pixels. Starting a measurement from the epithelial to the endothelial side, a series of en face images was obtained. From detected en face images, the epithelial cells, Bowman's layer, stromal keratocyte, nerve fiber, Descemet's membrane, and endothelial cell were clearly observed. Keratocyte cytoplasm, its nuclei, and its processes were also separately detected. Two-dimensional interconnectivity of the keratocytes is visualized, and the keratocytes existing between collagen lamellaes are separately extracted by exploiting a high axial resolution ability of FF-OCT.

Paper Details

Date Published: 1 July 2007
PDF: 7 pages
J. Biomed. Opt. 12(4) 041202 doi: 10.1117/1.2764461
Published in: Journal of Biomedical Optics Volume 12, Issue 4
Show Author Affiliations
Masahiro Akiba, Yamagata Promotional Organization for Industrial Technology (United States)
Naoyuki Maeda, Osaka Univ. (Japan)
Kazuhiko Yumikake, Osaka Univ. (Japan)
Takeshi Soma, Osaka Univ. (Japan)
Kohji Nishida, Tohoku Univ. (Japan)
Yasuo Tano, Osaka Univ. (Japan)
Kin-Pui Chan, Topcon Medical Systems, Inc. (United States)

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