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Journal of Biomedical Optics

Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells
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Paper Abstract

We present spectral domain phase microscopy (SDPM) as a new tool for measurements at the cellular scale. SDPM is a functional extension of spectral domain optical coherence tomography that allows for the detection of cellular motions and dynamics with nanometer-scale sensitivity in real time. Our goal was to use SDPM to investigate the mechanical properties of the cytoskeleton of MCF-7 cells. Magnetic tweezers were designed to apply a vertical force to ligand-coated magnetic beads attached to integrin receptors on the cell surfaces. SDPM was used to resolve cell surface motions induced by the applied stresses. The cytoskeletal response to an applied force is shown for both normal cells and those with compromised actin networks due to treatment with Cytochalasin D. The cell response data were fit to several models for cytoskeletal rheology, including one- and two-exponential mechanical models, as well as a power law. Finally, we correlated displacement measurements to physical characteristics of individual cells to better compare properties across many cells, reducing the coefficient of variation of extracted model parameters by up to 50%.

Paper Details

Date Published: 1 July 2007
PDF: 11 pages
J. Biomed. Opt. 12(4) 044008 doi: 10.1117/1.2753755
Published in: Journal of Biomedical Optics Volume 12, Issue 4
Show Author Affiliations
Emily J. McDowell, California Institute of Technology (United States)
Audrey K. Ellerbee, Duke Univ. (United States)
Michael A. Choma, Duke Univ. (United States)
Brian E. Applegate, Texas A&M Univ. (United States)
Joseph A. Izatt, Duke Univ. (United States)


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