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Journal of Biomedical Optics

Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm
Author(s): Huafeng Ding; Jun Q. Lu; Robert Scott Brock; Thomas J. McConnelll; Jenifer F. Ojeda; Kenneth Jacobs; Xin-Hua Hu
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Paper Abstract

Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system with a photoelastic modulation scheme to determine selected Mueller matrix elements of B-cell hydrosol samples. The angular dependence of S11, S12, and S34 is determined from the scattered light signals between 10 and 160 deg at the three wavelengths 442, 633, and 850 nm. A finite-difference, time-domain (FDTD) method and coated-sphere model are used to investigate the effect of nuclear refractive index on the angle-resolved Mueller elements at different wavelengths using the 3-D structures of selected B cells reconstructed from confocal images. With these results, we demonstrate the value of the light-scattering method in obtaining the cell morphology information.

Paper Details

Date Published: 1 May 2007
PDF: 9 pages
J. Biomed. Opt. 12(3) 034032 doi: 10.1117/1.2749730
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Huafeng Ding, East Carolina Univ. (United States)
Jun Q. Lu, East Carolina Univ. (United States)
Robert Scott Brock, East Carolina Univ. (United States)
Thomas J. McConnelll, East Carolina Univ. (United States)
Jenifer F. Ojeda, East Carolina Univ. (United States)
Kenneth Jacobs, East Carolina Univ. (United States)
Xin-Hua Hu, East Carolina Univ. (United States)

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