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Journal of Biomedical Optics • Open Access

Blood oxyhemoglobin saturation measurements by blue-green spectral shift
Author(s): Kurt R. Denninghoff; Russell A. Chipman; Lloyd W. Hillman

Paper Abstract

Previous work describing a resilient method for measuring oxyhemoglobin saturation using the blue-green spectral shift was performed using cell free hemoglobin solutions. Hemoglobin solution and whole blood sample spectra measured under similar conditions in a spectrophotometer are used here to begin evaluating the impact of cellular scattering on this method. The blue-green spectral shift with changing oxyhemoglobin saturation was preserved in these blood samples and the blue-green spectral shift was relatively unaffected by physiological changes in blood pH (6.6, 7.1, and 7.4), path length through blood (100 and 200 μm), and blood hematocrit (19 to 48%). The packaging of hemoglobin in red blood cells leads to a decreased apparent path length through hemoglobin, and an overall decrease in scattering loss with increasing wavelength from 450 to 850 nm. The negative slope of the scattering loss in the 476 to 516-nm range leads to a +3.0 nm shift in the oxyhemoglobin saturation calibration line when the blue-green spectral minimum in these blood samples was compared to cell free hemoglobin. Further research is needed to fully evaluate the blue green spectral shift method in cellular systems including in vivo testing.

Paper Details

Date Published: 1 May 2007
PDF: 5 pages
J. Biomed. Opt. 12(3) 034020 doi: 10.1117/1.2745312
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Kurt R. Denninghoff, The Univ. of Alabama at Birmingham (United States)
Russell A. Chipman, College of Optical Sciences/The Univ. of Arizona (United States)
Lloyd W. Hillman, The Univ. of Alabama in Huntsville (United States)

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