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Journal of Biomedical Optics

Combined magnetic resonance and bioluminescence imaging of live mice
Author(s): Mathieu Allard; Daniel Coté; Lorinda Davidson; Jun Dazai; Mark Henkelman
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Paper Abstract

We perform combined magnetic resonance and bioluminescence imaging of live mice for the purpose of improving the accuracy of bioluminescence tomography. The imaging is performed on three live nude mice in which tritium-powered light sources are surgically implanted. High-resolution magnetic resonance images and multispectral, multiview bioluminescence images are acquired in the same session. An anatomical model is constructed by segmenting the magnetic resonance images for all major tissues. The model is subsequently registered with nonlinear transformations to the 3-D light exittance (exiting intensity) surface map generated from the luminescence images. A Monte Carlo algorithm, along with a set of tissue optical properties obtained from in vivo measurements, is used to solve the forward problem. The measured and simulated light exittance images are found to differ by a factor of up to 2. The greatest cause of this moderate discrepancy is traced to the small errors in source positioning, and to a lesser extent to the optical properties used for the tissues. Discarding the anatomy and using a homogeneous model leads to a marginally worse agreement between the simulated and measured data.

Paper Details

Date Published: 1 May 2007
PDF: 11 pages
J. Biomed. Opt. 12(3) 034018 doi: 10.1117/1.2745298
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Mathieu Allard, Hospital for Sick Children (Canada)
Daniel Coté, Univ. Laval (Canada)
Lorinda Davidson, Hospital for Sick Children (Canada)
Jun Dazai, Hospital for Sick Children (Canada)
Mark Henkelman, Hospital for Sick Children (Canada)

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