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Journal of Biomedical Optics

Instrumentation as a source of variability in the application of fluorescence spectroscopic devices for detecting cervical neoplasia
Author(s): Brian M. Pikkula; Olga Shuhatovich; Roderick L. Price; Dan M. Serachitopol; Michelle Follen; Nick McKinnon; Calum E. MacAulay; Rebecca R. Richards-Kortum; Jong Soo Lee; Edward Neely Atkinson; Dennis D. Cox
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Paper Abstract

We report on a study designed to assess variability among three different fluorescence spectroscopy devices, four fiber optic probes, and three sets of optical calibration standards to better understand the reproducibility of measurements and interdevice comparisons of fluorescence spectroscopic data intended for clinical diagnostic use. Multiple measurements are acquired from all sets of standards using each combination of spectrometer, fiber optic probe, and optical standard. Data are processed using standard calibration methods to remove instrument-dependant responses. Processed spectra are analyzed using an analysis of variance to assess the percent variance explained by each factor that was statistically significant. Analysis of processed data confirms statistically significant differences among the spectrometers and fiber optic probes. However, no differences are found when varying calibration standards or measurement date and time. The spectrometers and fiber optic probes are significant sources of variability, but appropriate data processing substantially reduces these effects. Studies of inter- and intradevice variability are important methodological issues for optical device trials and must be included in the quality assurance studies for the clinical trial design.

Paper Details

Date Published: 1 May 2007
PDF: 11 pages
J. Biomed. Opt. 12(3) 034014 doi: 10.1117/1.2745285
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Brian M. Pikkula, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Olga Shuhatovich, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Roderick L. Price, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Dan M. Serachitopol, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Michelle Follen, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Nick McKinnon, BC Cancer Agency (Canada)
Calum E. MacAulay, BC Cancer Agency (Canada)
Rebecca R. Richards-Kortum, Rice Univ. (United States)
Jong Soo Lee, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Edward Neely Atkinson, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Dennis D. Cox, Rice Univ. (United States)


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