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Journal of Biomedical Optics

New statistical methods enhance imaging of cameleon fluorescence resonance energy transfer in cultured zebrafish spinal neurons
Author(s): Xiang Fan; Anirban Majumder; Sean S. Reagin; Erika L. Porter; Andrew T. Sornborger; Charles H. Keith; James D. Lauderdale
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Paper Abstract

Cameleons are genetically encoded fluorescence resonance energy transfer (FRET)–based Ca2+ indicators. Attempts to use cameleons to detect neural activity in vertebrate systems have been largely frustrated by the small FRET signal, in contradistinction to the higher signals seen in Drosophila and Caenorhabditis elegans. We have developed a statistical optimization method capable of detecting small ratiometric signals in noisy imaging data, called statistical optimization for the analysis of ratiometric signals. Using this method, we can detect and estimate anticorrelated ratiometric signals with subcellular resolution in cultured, dissociated zebrafish spinal neurons expressing cameleon or loaded with fluo-4 and fura-red. This method may make it possible to use yellow cameleons for measuring neural activity at high resolution in transgenic animals.

Paper Details

Date Published: 1 May 2007
PDF: 7 pages
J. Biomed. Opt. 12(3) 034017 doi: 10.1117/1.2745263
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Xiang Fan, Univ. of Georgia (United States)
Anirban Majumder, Univ. of Georgia (United States)
Sean S. Reagin, Univ. of Georgia (United States)
Erika L. Porter, The Univ. of Georgia (United States)
Andrew T. Sornborger, The Univ. of Georgia (United States)
Charles H. Keith, The Univ. of Georgia (United States)
James D. Lauderdale, The Univ. of Georgia (United States)


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