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Journal of Biomedical Optics

Quantitative comparison of tissue oxygen and motexafin lutetium uptake by ex vivo and noninvasive in vivo techniques in patients with intraperitoneal carcinomatosis
Author(s): Hsing-Wen Wang; Jarod C. Finlay; Kijoon Lee; Timothy C. Zhu; Mary P. Putt; Eli Glatstein; Cameron J. Koch; Sydney M. Evans; Stephen M. Hahn; Theresa M. Busch; Arjun G. Yodh
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Paper Abstract

(PDT). This technique has been extensively validated in tissue phantoms; however, validation in patients has been limited. This pilot study compares blood oxygenation and photosensitizer tissue uptake measured by multiwavelength DRS with ex vivo assays of the hypoxia marker, 2-(2-nitroimida-zol-1[H]-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide (EF5), and the photosensitizer (motexafin lutetium, MLu) from tissues at the same tumor site of three tumors in two patients with intra-abdominal cancers. Similar in vivo and ex vivo measurements of MLu concentration are carried out in murine radiation-induced fibrosarcoma (RIF) tumors (n=9). The selection of optimal DRS wavelength range and source-detector separations is discussed and implemented, and the association between in vivo and ex vivo measurements is examined. The results demonstrate a negative correlation between blood oxygen saturation (StO2) and EF5 binding, consistent with published relationships between EF5 binding and electrode measured pO2, and between electrode measured pO2 and StO2. A tight correspondence is observed between in vivo DRS and ex vivo measured MLu concentration in the RIF tumors; similar data are positively correlated in the human intraperitoneal tumors. These results further demonstrate the potential of in vivo DRS measurements in clinical PDT.

Paper Details

Date Published: 1 May 2007
PDF: 10 pages
J. Biomed. Opt. 12(3) 034023 doi: 10.1117/1.2743082
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Hsing-Wen Wang, National Yang-Ming Univ. (Taiwan)
Jarod C. Finlay, Univ. of Pennsylvania (United States)
Kijoon Lee, Univ. of Pennsylvania (United States)
Timothy C. Zhu, Univ. of Pennsylvania (United States)
Mary P. Putt, Univ. of Pennsylvania (United States)
Eli Glatstein, Univ. of Pennsylvania (United States)
Cameron J. Koch, Univ. of Pennsylvania (United States)
Sydney M. Evans, Univ. of Pennsylvania (United States)
Stephen M. Hahn, Univ. of Pennsylvania (United States)
Theresa M. Busch, Univ. of Pennsylvania (United States)
Arjun G. Yodh, Univ. of Pennsylvania (United States)

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