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Journal of Biomedical Optics

Damage thresholds for cultured retinal pigment epithelial cells exposed to lasers at 532 nm and 458 nm
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Paper Abstract

The determination of safe exposure levels for lasers has come from damage assessment experiments in live animals, which typically involve correlating visually identifiable damage with laser dosimetry. Studying basic mechanisms of laser damage in animal retinal systems often requires tissue sampling (animal sacrifice), making justification and animal availability problematic. We determined laser damage thresholds in cultured monolayers of a human retinal pigment epithelial (RPE) cell line. By varying exposure duration and laser wavelength, we identified conditions leading to damage by presumed photochemical or thermal mechanisms. A comparison with literature values for ocular damage thresholds validates the in vitro model. The in vitro system described will facilitate molecular and cellular approaches for understanding laser-tissue interaction.

Paper Details

Date Published: 1 May 2007
PDF: 8 pages
J. Biomed. Opt. 12(3) 034030 doi: 10.1117/1.2737394
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Michael L. Denton, Northrop Grumman Corp. (United States)
Michael S. Foltz, Northrop Grumman Corp. (United States)
Kurt J. Schuster, Northrop Grumman Corp. (United States)
Larry E. Estlack, Conceptual MindWorks, Inc. (United States)
Robert J. Thomas, Air Force Research Lab. (United States)


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