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Journal of Biomedical Optics

Non-contact detection of ciprofloxacin in a model anterior chamber using Raman spectroscopy
Author(s): Th. Sideroudi; Nikolaos Pharmakakis; A. Tyrovolas; G. Papatheodorou; G.D. Chryssikos; G.A. Voyiatzis
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Paper Abstract

We examine the application of an improved noncontact and noninvasive Raman spectroscopic technique in measuring medicines at therapeutic concentrations in a model system mimicking the anterior chamber of the eye. A 90-deg laser Raman scattering geometry is employed to reduce the direct exposure of the basic cordial ocular tissues to the laser beam and increase the signal-to-noise ratio of the spectra. The technique is applied to a commercially available artificial anterior chamber (AAC) fitted with corneas of porcine eyes. Specific Raman signatures of ciprofloxacin (Ciproxin®), a fluoroquinolone based antibiotic, have been resolved. Last, a partial least-squares (PLS) chemometric algorithm has been developed to predict the concentration of ciprofloxacin in AAC over the range from 0 to 1 mg/mL with a correlation coefficient R2=98.4% and an RMS error of prediction equal to 41 μg/mL.

Paper Details

Date Published: 1 May 2007
PDF: 6 pages
J. Biomed. Opt. 12(3) 034005 doi: 10.1117/1.2737385
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Th. Sideroudi, Foundation for Research and Technology-Hellas (Greece)
Nikolaos Pharmakakis, Univ. of Patras (Greece)
A. Tyrovolas, Univ. of Patras (Greece)
G. Papatheodorou, Univ. of Patras (Greece)
G.D. Chryssikos, National Hellenic Research Foundation (Greece)
G.A. Voyiatzis, Foundation for Research and Technology-Hellas (Greece)

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