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Journal of Biomedical Optics

Plasmon resonance coupling of metal nanoparticles for molecular imaging of carcinogenesis in vivo
Author(s): Jesse S. Aaron; Nitin Nitin; Kort Travis; Sonia Kumar; Thomas G. Collier; Sun Young Park; Miguel Jose Yacaman; Lezlee Coghlan; Michelle Follen; Rebecca R. Richards-Kortum; Konstantin V. Sokolov
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Paper Abstract

An effective cancer control strategy requires improved early detection methods, patient-specific drug selection, and the ability to assess response to targeted therapeutics. Recently, plasmon resonance coupling between closely spaced metal nanoparticles has been used to develop ultrasensitive bioanalytical assays in vitro. We demonstrate the first in vivo application of plasmon coupling for molecular imaging of carcinogenesis. We describe molecular-specific gold bioconjugates to image epidermal growth factor receptor (EGFR); these conjugates can be delivered topically and imaged noninvasively in real time. We show that labeling with gold bioconjugates gives information on the overexpression and nanoscale spatial relationship of EGF receptors in cell membranes, both of which are altered in neoplasia. EGFR-mediated aggregation of gold nanoparticles in neoplastic cells results in more than a 100-nm color shift and a contrast ratio of more than tenfold in images of normal and precancerous epithelium in vivo, dramatically increasing contrast beyond values reported previously for antibody-targeted fluorescent dyes.

Paper Details

Date Published: 1 May 2007
PDF: 11 pages
J. Biomed. Opt. 12(3) 034007 doi: 10.1117/1.2737351
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Jesse S. Aaron, The Univ. of Texas at Austin (United States)
Nitin Nitin, Georgia Institute of Technology (United States)
Kort Travis, The Univ. of Texas at Austin (United States)
Sonia Kumar, The Univ. of Texas at Austin (United States)
Thomas G. Collier, The Univ. of Texas at Austin (United States)
Sun Young Park, The Univ. of Texas at Austin (United States)
Miguel Jose Yacaman, The Univ. of Texas at Austin (United States)
Lezlee Coghlan, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Michelle Follen, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)
Konstantin V. Sokolov, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)


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