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Journal of Biomedical Optics

Scattering suppression and confocal detection in multifocal multiphoton microscopy
Author(s): Jörg Martini; Volker Andresen; Dario Anselmetti
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Paper Abstract

We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality.

Paper Details

Date Published: 1 May 2007
PDF: 6 pages
J. Biomed. Opt. 12(3) 034010 doi: 10.1117/1.2736425
Published in: Journal of Biomedical Optics Volume 12, Issue 3
Show Author Affiliations
Jörg Martini
Volker Andresen, LaVision BioTec GmbH (Germany)
Dario Anselmetti, Univ. Bielefeld (Germany)

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