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Journal of Biomedical Optics • Open Access

Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells
Author(s): Steven Boutrus; Cherry Anne Greiner; Derrick Hwu; Michael Chan; Charlotte Kuperwasser; Charles P. Lin; Irene Georgakoudi

Paper Abstract

The recent introduction of the in vivo flow cytometer for real-time, noninvasive detection and quantification of cells circulating in the vasculature of small animals has provided a powerful tool for tracking the roles of different types of cells in disease progression. We describe a portable version of the device, which provides the capability to: a) excite and detect fluorescence at two distinct colors simultaneously, and b) perform data analysis in real time. These advances improve significantly the utility of the instrument and provide a means of increasing detection specificity. As examples, we present the depletion kinetics of circulating green fluorescent protein (GFP)-labeled breast cancer cells in the vasculature of mice, and the specific detection of circulating hematopoietic stem cells labeled in vivo with two antibodies

Paper Details

Date Published: 1 March 2007
PDF: 3 pages
J. Biomed. Opt. 12(2) 020507 doi: 10.1117/1.2722733
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Steven Boutrus, Tufts Univ. (United States)
Cherry Anne Greiner, Tufts Univ. (United States)
Derrick Hwu, Tufts Univ. (United States)
Michael Chan, Tufts-New England Medical Ctr. (United States)
Charlotte Kuperwasser, Tufts Univ. School of Medicine (United States)
Charles P. Lin, Massachusetts General Hospital (United States)
Irene Georgakoudi, Tufts Univ. (United States)

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