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Journal of Biomedical Optics

Mirror slides for high-sensitivity cell and tissue fluorescence imaging
Author(s): Eric Le Moal; Emmanuel Fort; Sandrine Lévêque-Fort; Anne Janin; Hideyuki Murata; Fabrice P. Cordelieres; Marie-Pierre P. Fontaine-Aupart; Christian Ricolleau
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Paper Abstract

Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.

Paper Details

Date Published: 1 March 2007
PDF: 5 pages
J. Biomed. Opt. 12(2) 024030 doi: 10.1117/1.2722730
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Eric Le Moal, École Supérieure de Physique et de Chimie Industrielles (France)
Emmanuel Fort, École Supérieure de Physique et de Chimie Industrielles (France)
Sandrine Lévêque-Fort, Univ. Paris-Sud II (France)
Anne Janin, Univ. Paris VII (France)
Hideyuki Murata, Univ. Paris VII (France)
Fabrice P. Cordelieres, Institut Curie (France)
Marie-Pierre P. Fontaine-Aupart, Univ. Paris-Sud II (France)
Christian Ricolleau, École Supérieure de Physique et de Chimie Industrielles (France)


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