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Journal of Biomedical Optics • Open Access

Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells
Author(s): Veronica Gomez-Godinez; Nicole Wakida; Alexander S. Dvornikov; Kyoko Yokomori; Michael W. Berns

Paper Abstract

An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of γH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.

Paper Details

Date Published: 1 March 2007
PDF: 3 pages
J. Biomed. Opt. 12(2) 020505 doi: 10.1117/1.2717684
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Veronica Gomez-Godinez, Univ. of California/Irvine (United States)
Nicole Wakida, Univ. of California/Irvine (United States)
Alexander S. Dvornikov, Univ. of California/Irvine (United States)
Kyoko Yokomori, Univ. of California/Irvine (United States)
Michael W. Berns, Univ. of California/Irvine (United States)

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