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Journal of Biomedical Optics • Open Access

Expansion of intensity correlation spectroscopy for lifetime measurements—application to intracellular oxygen dynamics measurements
Author(s): Goro Nishimura; Changi Pack; Mamoru Tamura

Paper Abstract

We report on a simple correlation method for lifetime measurements using a random modulated excitation light source. We use an intensity correlation function of emission for lifetime analyses. In this method, no reference timing of the excitation is required. We apply the correlation method to measure phosphorescence decays and successfully demonstrate in the analysis of the phosphorescence decay from Pd(II) porphine in HeLa cells under aerobic and anaerobic conditions to understand the oxygen dynamics in individual cells. The method is applicable to faster decay time measurements down to a nanosecond range when the detection system is improved. Current fluorescence correlation setups can easily be modified for lifetime measurements, expanding the applicability in biological problems.

Paper Details

Date Published: 1 March 2007
PDF: 3 pages
J. Biomed. Opt. 12(2) 020503 doi: 10.1117/1.2717623
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Goro Nishimura, Hokkaido Univ. (Japan)
Changi Pack, Hokkaido Univ. (Japan)
Mamoru Tamura, Hokkaido Univ. (Japan)

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