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Journal of Biomedical Optics • Open Access

Photoacoustic imaging of lacZ gene expression in vivo
Author(s): Li Li; Roger J. Zemp; Gina F. Lungu; George Stoica; Lihong V. Wang

Paper Abstract

In the postgenomic era, imaging techniques are playing an important role in visualizing gene expression in vivo. This work represents the first demonstration of photoacoustic tomography (PAT) for reporter gene imaging. Rats inoculated with 9L/lacZ gliosarcoma tumor cells are imaged with PAT before and after injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme β-galactosidase. Using far-red optical illumination, the genetically tagged tumors in rats are clearly visualized by PAT following the assay. The spatial resolution is quantified to be less than 400 μm, while 500-nM-level sensitivity is demonstrated. With the future development of new absorption-based reporter gene systems, it is anticipated that photoacoustic technology will provide a valuable tool for molecular imaging research.

Paper Details

Date Published: 1 March 2007
PDF: 3 pages
J. Biomed. Opt. 12(2) 020504 doi: 10.1117/1.2717531
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Li Li, Washington Univ. in St. Louis (United States)
Roger J. Zemp, Washington Univ. in St. Louis (United States)
Gina F. Lungu, Texas A&M Univ. (United States)
George Stoica, Texas A&M Univ. (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


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