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Journal of Biomedical Optics

Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
Author(s): Kristen E. Adams; Shi Ke; Sunkuk Kwon; Feng Liang; Zhen Fan; Yang Lu; Karen Hirshi; Michel E. Mawad; Michael A. Barry; Eva Marie Sevick-Muraca
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Paper Abstract

Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially can provide large signal-to-noise ratios (SNRs) for image detection of target tissues. In the literature, many small animal imaging studies are performed with a red excitable dye, Cy5.5, conjugated to the targeting component. We report the comparison of the in vivo fluorescent imaging performance of a near-IR (NIR) and a red-excitable dye. Epidermal growth factor (EGF) was conjugated with Cy5.5 [excitation/emission (ex/em), 660/710 nm] or IRDye® 800CW (ex/em: 785/830 nm) for imaging EGF receptor (EGFr) positive (MDA-MB-468) and/or negative (MDA-MB-435) human breast cancer cell lines in subcutaneous xenograft models. The conjugates were injected intravenously at 1-nmol-dye equivalent with and without anti-EGFr monoclonal antibody C225, preadministered 24 h prior as a competitive ligand to EGFr. Our images show that while both agents target EGFr, the EGF-IRDye® 800CW evidenced a significantly reduced background and enhanced the tumor-to-background ratio (TBR) compared to the EGF-Cy5.5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered.

Paper Details

Date Published: 1 March 2007
PDF: 9 pages
J. Biomed. Opt. 12(2) 024017 doi: 10.1117/1.2717137
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Kristen E. Adams, Baylor College of Medicine (United States)
Shi Ke, Baylor College of Medicine (United States)
Sunkuk Kwon, Baylor College of Medicine (United States)
Feng Liang, Baylor College of Medicine (United States)
Zhen Fan, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Yang Lu, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Karen Hirshi, Baylor College of Medicine (United States)
Michel E. Mawad, Baylor College of Medicine (United States)
Michael A. Barry, Baylor College of Medicine (United States)
Eva Marie Sevick-Muraca, Baylor College of Medicine (United States)


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