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Journal of Biomedical Optics

Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis
Author(s): Hsian-Yuan Tan; Yen Sun; Wen Lo; Shu-Wen Teng; Ruei-Jr Wu; Shiou-Hwa Jee; Wei-Chou Lin; Ching-Hsi Hsiao; Hsin-Chiung Lin; Yeong-Fong Chen; David Hui-Kang Ma; Samuel Chao-Ming Huang; Sung-Jan Lin; Chen-Yuan Dong
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Paper Abstract

The purpose of this study is to demonstrate the application of multiphoton fluorescence and second harmonic generation (SHG) microscopy for the ex-vivo visualization of human corneal morphological alterations due to infectious processes. The structural alterations of both cellular and collagenous components can be respectively demonstrated using fluorescence and SHG imaging. In addition, pathogens with fluorescence may be identified within turbid specimens. Our results show that multiphoton microscopy is effective for identifying structural alterations due to corneal infections without the need of histological processing. With additional developments, multiphoton microscopy has the potential to be developed into an imaging technique effective in the clinical diagnosis and monitoring of corneal infections.

Paper Details

Date Published: 1 March 2007
PDF: 8 pages
J. Biomed. Opt. 12(2) 024013 doi: 10.1117/1.2717133
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Hsian-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Shu-Wen Teng, National Taiwan Univ. (Taiwan)
Ruei-Jr Wu, National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital (Taiwan)
Wei-Chou Lin, National Taiwan Univ. Hospital (Taiwan)
Ching-Hsi Hsiao, Chang Gung Memorial Hospital (Taiwan)
Hsin-Chiung Lin, Chang Gung Memorial Hospital (Taiwan)
Yeong-Fong Chen, Chang Gung Memorial Hospital (Taiwan)
David Hui-Kang Ma, Chang Gung Memorial Hospital (Taiwan)
Samuel Chao-Ming Huang, Chang Gung Memorial Hospital (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

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