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Journal of Biomedical Optics

Analysis of the dispersion compensation of acousto-optic deflectors used for multiphoton imaging
Author(s): Shaoqun Zeng; Xiaohua Lv; Kun Bi; Chen Zhan; Derong Li; Wei R. Chen; Wenhui Xiong; Steven L. Jacques; Qingming Luo
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Paper Abstract

The acousto-optic deflector (AOD) is highly preferred in laser scanning microscopy for its fast scanning ability and random-addressing capability. However, its application in two-photon microscopy is frustrated by the dispersion of the AOD, which results in beam distortion and pulse lengthening. We report the analysis of simultaneous compensation for the angular dispersion and temporal dispersion of the AOD by merely introducing a single dispersive element such as a prism or a grating. Besides serving as a scanner, the AOD is also a part of the compressor pair by integrating the dispersive nature of the AO interaction. This compensation principle is effective for both one-dimensional (1-D) AOD and two-dimensional (2-D) AOD scanning. Switching from a 1-D to a 2-D system requires proper optical alignment with the compensation element, but does not involve any new components. Analytical expressions are given to illustrate the working principle and to help with understanding the design of the system. Fluorescence images of beads and cells are shown to demonstrate the performance of two-photon microscopy when applying this compensated 2-D AOD as scanner.

Paper Details

Date Published: 1 March 2007
PDF: 7 pages
J. Biomed. Opt. 12(2) 024015 doi: 10.1117/1.2714061
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Shaoqun Zeng, Yale Univ. (United States)
Xiaohua Lv, Huazhong Univ. of Science and Technology (China)
Kun Bi, Huazhong Univ. of Science and Technology (China)
Chen Zhan, Huazhong Univ. of Science and Technology (China)
Derong Li, Huazhong Univ. of Science and Technology (China)
Wei R. Chen, Yale Univ. (United States)
Wenhui Xiong, Yale Univ. (United States)
Steven L. Jacques, Oregon Health and Science Univ. (United States)
Qingming Luo, Huazhong Univ. of Science and Technology (China)

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