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Journal of Biomedical Optics

Fiber optic biosensor for the detection of C-reactive protein and the study of protein binding kinetics
Author(s): Chien Chou; Hsien-Yeh Hsu; Hsieh-Ting Wu; Kai-Yu Tseng; Arthur E. T. Chiou; Chih-Jen Yu; Zheng-Yuan Lee; Tsu-Shin Chan
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Paper Abstract

Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase protein belonging to pentraxin family, and an isoform of pCRP, modified CRP (mCRP), have been suggested to have proinflammation effects on vascular cells in acute myocardial infarction (AMI). In current studies, we generate mCRP from pCRP, and use several methods including fluorescence spectral properties, circular dichroism, analytical ultracentrifuge, and Western blotting to demonstrate their differences in physical and chemical properties as well as the purity of pCRP and mCRP. In addition, we design and implement an FOB to study the real-time qualitative and quantitative biomolecular recognition of CRP isoforms. Specifically, the association and dissociation rate constants of the reaction between FITC-conjugated monoclonal sheep anti-human CRP antibody and the pCRP and mCRP are determined. The feasibility of our current approach to measure the association and dissociation rate constants of the reaction between tested CRP isoforms was successfully demonstrated.

Paper Details

Date Published: 1 March 2007
PDF: 9 pages
J. Biomed. Opt. 12(2) 024025 doi: 10.1117/1.2714029
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Chien Chou, National Yang-Ming Univ. (Taiwan)
Hsien-Yeh Hsu, National Yang-Ming Univ. (Taiwan)
Hsieh-Ting Wu, National Yang-Ming Univ. (Taiwan)
Kai-Yu Tseng, National Yang-Ming Univ. (Taiwan)
Arthur E. T. Chiou, National Yang-Ming Univ. (Taiwan)
Chih-Jen Yu, National Central Univ. (Taiwan)
Zheng-Yuan Lee, National Yang-Ming Univ. (Taiwan)
Tsu-Shin Chan, National Yang-Ming Univ. (Taiwan)

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