Share Email Print
cover

Journal of Biomedical Optics

Visualization of hepatobiliary excretory function by intravital multiphoton microscopy
Author(s): Yuan Liu; Hsiao-Ching Chen; Shu-Mei Yang; Tzu-Lin Sun; Wen Lo; Ling-Ling Chiou; Guan-Tarn Huang; Chen-Yuan Dong; Hsuan-Shu Lee
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an intravital hepatic imaging chamber. The high-quality images show sequential uptake and processing of 6-CFDA from the hepatocytes and the subsequent excretion into bile canaliculi within approximately 50 min. This is a promising technique to study intravital hepatic physiology and metabolism.

Paper Details

Date Published: 1 January 2007
PDF: 5 pages
J. Biomed. Opt. 12(1) 014014 doi: 10.1117/1.2710237
Published in: Journal of Biomedical Optics Volume 12, Issue 1
Show Author Affiliations
Yuan Liu, National Taiwan Univ. (Taiwan)
Hsiao-Ching Chen, National Taiwan Univ. Hospital (Taiwan)
Shu-Mei Yang, National Taiwan Univ. Hospital (Taiwan)
Tzu-Lin Sun, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Ling-Ling Chiou, National Taiwan Univ. Hospital (Taiwan)
Guan-Tarn Huang, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)


© SPIE. Terms of Use
Back to Top