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Journal of Biomedical Optics

Visualization of hepatobiliary excretory function by intravital multiphoton microscopy
Author(s): Yuan Liu; Hsiao-Ching Chen; Shu-Mei Yang; Tzu-Lin Sun; Wen Lo; Ling-Ling Chiou; Guan-Tarn Huang; Chen-Yuan Dong; Hsuan-Shu Lee
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Paper Abstract

Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an intravital hepatic imaging chamber. The high-quality images show sequential uptake and processing of 6-CFDA from the hepatocytes and the subsequent excretion into bile canaliculi within approximately 50 min. This is a promising technique to study intravital hepatic physiology and metabolism.

Paper Details

Date Published: 1 January 2007
PDF: 5 pages
J. Biomed. Opt. 12(1) 014014 doi: 10.1117/1.2710237
Published in: Journal of Biomedical Optics Volume 12, Issue 1
Show Author Affiliations
Yuan Liu, National Taiwan Univ. (Taiwan)
Hsiao-Ching Chen, National Taiwan Univ. Hospital (Taiwan)
Shu-Mei Yang, National Taiwan Univ. Hospital (Taiwan)
Tzu-Lin Sun, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Ling-Ling Chiou, National Taiwan Univ. Hospital (Taiwan)
Guan-Tarn Huang, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)

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