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Journal of Biomedical Optics

Three-dimensional imaging of whole rodent organs using optical computed and emission tomography
Author(s): Mark Oldham; H. Sakhalkar; Y. M. Wang; P. Guo; Tom F. Oliver; R. Bentley; Zeljko Vujaskovic; Mark W. Dewhirst
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Paper Abstract

We explore the potential of optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) in a new area—whole organ imaging. The techniques are implemented on an in-house prototype benchtop system with improved image quality and the capacity to image larger samples (up to 3 cm) than previous systems based on stereo microscopes. Imaging performance tests confirm high geometrical accuracy, accurate relative measurement of linear attenuation coefficients, and the ability to image features at the 50-µm level. Optical labeling of organ microvasculature was achieved using two stains deposited via natural in vivo circulatory processes: a passive absorbing ink-based stain and an active fluorescin FITC-lectin conjugate. The lectin protein binds to the endothelial lining, and FITC fluorescense enables optical-ECT imaging. Three-dimensional (3-D) optical-CT images have been acquired of a normal rat heart and left lung and a mouse right lung showing exquisite detail of the functional vasculature and relative perfusion distribution. Coregistered optical-ECT images were also acquired of the mouse lung and kidney. Histological sections confirmed effective labeling of microvasculature throughout the organs. The advantages of optical-CT and optical-ECT include the potential for a unique combination of high resolution and high contrast and compatibility with a wide variety of optical probes, including gene expression labeling fluorescent reporter proteins.

Paper Details

Date Published: 1 January 2007
PDF: 10 pages
J. Biomed. Opt. 12(1) 014009 doi: 10.1117/1.2709858
Published in: Journal of Biomedical Optics Volume 12, Issue 1
Show Author Affiliations
Mark Oldham, Duke Univ. (United States)
H. Sakhalkar, Duke Univ. (United States)
Y. M. Wang, Duke Univ. (United States)
P. Guo, Duke Univ. (United States)
Tom F. Oliver, Duke Univ. (United States)
R. Bentley, Duke Univ. (United States)
Zeljko Vujaskovic, Duke Univ. Medical Ctr. (United States)
Mark W. Dewhirst, Duke Univ. (United States)

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